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	<title>Equine Reproduction Services</title>
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		<title>KID EASY BAR</title>
		<link>http://www.frozen-semen.com/site/horses/kid-easy-bar</link>
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		<pubDate>Mon, 09 May 2011 16:45:45 +0000</pubDate>
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				<category><![CDATA[Horses]]></category>

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		<description><![CDATA[In Kid Easy Bar&#8217;s early career he was a champion working cow horse in the midwest Stallion Contract Shipped Semen Contract Transport Agreement Breeding Agreement Checklist]]></description>
				<content:encoded><![CDATA[<p>In Kid Easy Bar&#8217;s early career he was a champion working cow horse in the midwest</p>
<ul>
<li><a href="http://www.frozen-semen.com/site/wp-content/uploads/2013/03/2013-Kid-Easy-Bar.pdf">Stallion Contract</a></li>
<li><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/Semen_Request_Form.pdf">Shipped Semen Contract</a></li>
<li><a href="http://www.frozen-semen.com/site/wp-content/uploads/2013/03/2013-transported-semen-agreement.pdf">Transport Agreement</a></li>
<li><a href="http://www.frozen-semen.com/site/wp-content/uploads/2013/03/2013-CheckList.pdf">Breeding Agreement Checklist</a></li>
</ul>
<p><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/Easy-website-size.jpg"><img class="alignnone size-medium wp-image-222" title="Easy website size" alt="" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/Easy-website-size-300x240.jpg" width="300" height="240" /></a><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/IMG_4577sand.jpg"><img class="alignnone size-medium wp-image-223" title="IMG_4577sand" alt="" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/IMG_4577sand-240x300.jpg" width="240" height="300" /></a><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/IMG_453117bkg.jpg"><img class="alignnone size-medium wp-image-225" title="IMG_4531#17bkg" alt="" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/IMG_453117bkg-214x300.jpg" width="214" height="300" /></a></p>
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		<title>SAMPSON</title>
		<link>http://www.frozen-semen.com/site/horses/sampson</link>
		<comments>http://www.frozen-semen.com/site/horses/sampson#comments</comments>
		<pubDate>Mon, 09 May 2011 16:47:07 +0000</pubDate>
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				<category><![CDATA[Horses]]></category>

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		<description><![CDATA[89&#8242; Brown KWPN Stallion Sampson owned by esteemed international rider Kate Levy of Kate Levy LLC Stallion Contract Shipped Semen Contract Transport Agreement Visit Sampson&#8217;s Website www.kwpnsampson.com]]></description>
				<content:encoded><![CDATA[<p>89&#8242; Brown KWPN Stallion Sampson owned by esteemed international rider Kate Levy of Kate Levy LLC</p>
<ul>
<li><a href="http://www.frozen-semen.com/site/wp-content/uploads/2011/05/Sampson_breeding_contract1.pdf">Stallion Contract</a></li>
<li><a href="http://www.frozen-semen.com/site/wp-content/uploads/2011/05/Semen_Request_Form1.pdf">Shipped Semen Contract</a></li>
<li><a href="http://www.frozen-semen.com/site/wp-content/uploads/2011/05/Transport_-Agreement_AddendumA.pdf">Transport Agreement</a></li>
</ul>
<p>Visit Sampson&#8217;s Website</p>
<p><a href="http://www.kwpnsampson.com">www.kwpnsampson.com</a></p>
<p><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/IMG_6718.jpg"><img class="size-medium wp-image-210 alignnone" title="IMG_6718" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/IMG_6718-300x239.jpg" alt="" width="300" height="239" /></a><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/Sampson1A.jpg"><img class="alignnone size-medium wp-image-280" title="Sampson1A" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/Sampson1A-300x196.jpg" alt="" width="300" height="196" /></a><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/Sampson3a.jpg"><img class="alignnone size-medium wp-image-281" title="Sampson3a" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/Sampson3a-300x212.jpg" alt="" width="300" height="212" /></a></p>
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		<title>Memarax</title>
		<link>http://www.frozen-semen.com/site/horses/memarax</link>
		<comments>http://www.frozen-semen.com/site/horses/memarax#comments</comments>
		<pubDate>Wed, 11 May 2011 21:08:31 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Horses]]></category>

		<guid isPermaLink="false">http://clured.com/ph/ers/site/?p=250</guid>
		<description><![CDATA[92&#8242; Bay Arabian Stallion Winner of multiple halter futurities and most classic]]></description>
				<content:encoded><![CDATA[<p>92&#8242; Bay Arabian Stallion</p>
<p>Winner of multiple halter futurities and most classic</p>
<p><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/mem3.png"><img class="alignnone size-full wp-image-252" title="mem3" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/mem3.png" alt="" width="230" height="289" /></a><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/mem41.png"><img class="alignnone size-full wp-image-256" title="mem4" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/mem41.png" alt="" width="226" height="289" /></a></p>
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		<title>Tamerax</title>
		<link>http://www.frozen-semen.com/site/horses/tamerax</link>
		<comments>http://www.frozen-semen.com/site/horses/tamerax#comments</comments>
		<pubDate>Wed, 11 May 2011 23:32:55 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Horses]]></category>

		<guid isPermaLink="false">http://clured.com/ph/ers/site/?p=261</guid>
		<description><![CDATA[87&#8242; Bay Russian/Polish Arabian Stallion Versatile multiple champion halter, western pleasure, hunt seat, most classic, youth, armature and open.  Sire of champion endurance horse Silver Xerox &#38; halter champion Memarax Grandson of the immortal Arax]]></description>
				<content:encoded><![CDATA[<p>87&#8242; Bay Russian/Polish Arabian Stallion</p>
<p>Versatile multiple champion halter, western pleasure, hunt seat, most classic, youth, armature and open.  Sire of champion endurance horse Silver Xerox &amp; halter champion Memarax</p>
<p>Grandson of the immortal Arax</p>
<p><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/aip-file-078-website.jpg"><img class="alignnone size-medium wp-image-285" title="aip file 078 website" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/aip-file-078-website-240x300.jpg" alt="" width="240" height="300" /></a><a href="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/tam.jpg"><img class="alignnone size-medium wp-image-286" title="tam" src="http://clured.com/ph/ers/site/wp-content/uploads/2011/05/tam-200x300.jpg" alt="" width="200" height="300" /></a></p>
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		<title>Phantoms: Breeding Mount Education for Safety and Success</title>
		<link>http://www.frozen-semen.com/site/articles/phantoms-breeding-mount-education-for-safety-and-success</link>
		<comments>http://www.frozen-semen.com/site/articles/phantoms-breeding-mount-education-for-safety-and-success#comments</comments>
		<pubDate>Fri, 13 May 2011 21:55:37 +0000</pubDate>
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				<category><![CDATA[Articles]]></category>

		<guid isPermaLink="false">http://184.172.187.61/~frozense/site/?p=333</guid>
		<description><![CDATA[Click Here to Order Your Phantom! Breeding mounts, also known as phantoms or dummies, are an integral part of a modern breeding operation. They serve as a substitute for a live mare, when collecting semen in an artificial insemination program. This includes inseminating on farm, and shipping cooled or frozen semen. Additionally, stallions may need [...]]]></description>
				<content:encoded><![CDATA[<p style="text-align: center;"><strong><a href="http://www.horsemansmall.com/ers.cfm?page=list.cfm&amp;criteria=25&amp;action=list&amp;cat=25&amp;startrow=1&amp;maxrows=10&amp;mem_ID=&amp;afid=PHJ&amp;">Click Here to Order Your Phantom!</a></strong></p>
<p>Breeding mounts, also known as phantoms or dummies, are an integral part of a modern breeding operation. They serve as a substitute for a live mare, when collecting semen in an artificial insemination program. This includes inseminating on farm, and shipping cooled or frozen semen. Additionally, stallions may need to be collected for semen evaluation and analysis, for pre season check ups, sales or insurance purposes. Most importantly, breeding phantoms greatly reduce the chance of injury to the stallion during semen collection. Another plus is that phantoms can be thoroughly cleaned and disinfected between mounts, minimizing transmission of disease and potential pathogens.</p>
<p>A properly designed phantom is of utmost importance in relation to the quality of the ejaculate. A stallion must be comfortable, pain free, and a full partner in the collection process. Stallions have the unique ability to ejaculate and withhold sperm. Causes may include poor collection procedures such as, artificial vaginas too hot or cold, wrong pressure, poor technician technique, or uncomfortable height, angle or width of the phantom. I have witnessed stallions so apparently angry with the collector, mount, or situation, that they ejaculate volumes of seminal fluids, with little to no sperm cells. When the situation changed, their ejaculates returned to normal. Stallions with uncomfortable collection memory, often do this, and can also become accumulators. This is a situation that causes a backup of sperm cells, possible blockage and occasionally hemospermia, which is blood in the semen.</p>
<p>Phantoms may be either home made or commercial, and are constructed in different styles. Most home made models are two legged, and are not adjustable by height or angle. This can create several problems, while being perfectly suitable for some stallions. The two legged design has been known to cause injury to the front legs of some stallions. Also, the collector must be able to position him/herself in such a fashion, so as to get the most satisfactory response from the stallion. You want your left foot, assuming you are collecting from the near side of the horse, behind the right, and close to the phantom. I have seen mounts built with the back post at the very end of the body of the mount. One stallion while thrusting, repeatedly hit the post with his back hooves, rendering him sore. Remember, not all stallions collect quietly. Some move around and will attempt to move up or cross to the side. His legs should not be subject to possible injury. Therefore a single post, set sufficiently back, is highly recommended. I have witnessed stallions with the configuration of double post, learn to mount and thrust from a far back position. This does not give him the acupressure support he needs, with his chest against the mount, for a strong ejaculation. Thereby, giving a lesser quality collection than he was capable of. Most stallions prefer only a slight degree of angle. The height recommended is slightly shorter at mid body then the height of the stallion. Again, adjustment may be necessary, taking into consideration the preference, age and/or physical limitations of the stallion.</p>
<p>Width of the mount is also be vital, but shape is seldom considered by builders. When building a home made phantom, it is often suggested to use an old water heater or log. Cover it with foam and vinyl, and &#8220;voila,&#8221; a breeding mount. Here is where this story goes wrong. These objects being round, are widest at the farthest point, east and west. Watch a stallion breed naturally. Upon mounting, he grips the mare in front of her hips and pelvic area. Notice this area on your mare. Nature has indented her here, and it is her least round, and most narrow area. Because &#8230; when a stallion ejaculates, he concurrently straightens and tenses his forelegs. He needs to do this to totally ejaculate. A breeding mount of round proportions, does not allow his forelegs to fall straight, thus straining his shoulders, forearms, and neck, as he tenses to ejaculate. A stallion who does not have a heavy or regular breeding schedule may not show signs of distress. On the other hand, we often hear of stallions with heavy collection schedules, needing a chiropractor. Not realizing the source of the problem, the stallion manager may believe the stallion unable to breed and still compete. He may just be sore from the unnatural position. If the condition causing the discomfort persists and is not recognized, the stallion may begin to dismount prematurely, or during ejaculation. This is a frustrating experience for both collector and stallion.</p>
<p>Remember too, that breeds of different stature and weight, are not dissimilar, as to the space between their forelegs. Just because your stallion is 17 hands, does not make him wider between the legs then a 14.2 Arabian. He may even be narrower. He does not need a wide mount. Many commercial mounts are also too wide for many breeds. Pay attention to this if constructing your own. I recommend 22 inches for standard horse breeds, and 24 for drafts. Too narrow can also cause discomfort.</p>
<p>This differs from weight bearing, and structural integrity. The stallion must never feel insecure of his weight on the mount. Again, a 15.2 Shire will weigh more then a 16.2 thoroughbred. The phantom must be built to be secure of his weight. Have you noticed a stallion pasture breeding, testing the weight bearing and intention of the mare? They will partially mount a number of times, and use their neck as a weight, to test the waters. This posturing will be imitated upon covering the phantom. Make certain phantom is secured firmly, and the body of the mount does not give, and can totally support the stallion&#8217;s weight while thrusting. Foam padding and a non abrasive cover should be of sufficient depth and density, to further add to his comfort.</p>
<p>Another area of wide debate, are mounts with internal orifices. In these models the stallion actually enters the mount, which is equipped with an internal artificial vagina. As the stallion ejaculates, the handler must use a lever to lower the artificial vagina&#8217;s angle. This allows the semen to descend into the filter and bottle. Timing here is imperative. There have been validated accounts of stallions being injured, and some seriously impaired by this method of non manual collection. While some stallions adjust well to this form of collection mount, without ever a hint of a problem, it is not recommended by many Universities and Reproduction vets. If purchasing such a unit used, and in doubt, simply cover the orifice so the stallion cannot enter, and proceed to collect with a hand held artificial vagina. As to any argument that this form of mount is a one man method, I have personally come to this conclusion. A bad stallion always needs more then one person present. A well mannered, stallion, can be handled and collected by the same person, using any mount, without any difference. Libido, temperament and training of the stallion, along with your own experience and ability, are serious considerations before attempting any collection procedures by oneself.</p>
<p>The actual training of the stallion to mount a phantom is not difficult. Patience, as in any form of training and conditioning, is paramount. One needs first to evaluate the stallion&#8217;s past breeding experience, libido, and physical ability to mount. I have not seen much difference training sexually unused stallions to aged veterans, as to their willingness to mount a phantom. Libido seems to equal out experience. One area where I have had to test my patience is professionally trained and shown Halter horses. They are firmly conditioned to exhibit no libido or sexual interest while in a halter with a chain. Many will turn away from a mare in estrus beckoning to him. Past experiences with dropping or vocalizing, have earned him a sharp reckoning. One way to get past this is a soft, heavy cotton lead, with a strong bull snap. It signals a non work climate. A pleasant alternative. I have also had great luck with, and recommend ,the use of a bright colored halter, different from their usual. All stallions soon come to recognize this as a signal of what is to come. This is similar to the Pavlov dog theory.</p>
<p>I prefer to initially use a mare in full heat, for phantom training. Some stallions can tease to a mare in stocks, a close visual stimulant, and be aroused enough to mount the phantom. This works particularly well with stallions experienced in live cover. If no stocks are available, or the stallion needs the mare to be in closer proximity, be certain you have an experienced mare handler/horse person. I like to scent the phantom with mare urine. Preferably the liquid expelled as the mare breaks down for the stallion. I have also used soiled shavings from an estral mare, rubbed onto the mount. This can be a powerful aphrodisiac. Next place the mare beside the phantom, her posterior at the business end of the mount. A sturdy phantom should allow the stallion to approach from the near side, and tease the mare. When dropped, guide the stallion to the back of the mount, placing his chest at a slight angle, into the near corner. In a state of excitement, when feeling the pressure of the phantom at his chest, his impulse will be to mount. The first sign I have that the stallion is progressing, is the rubbing of his head and neck on the phantom. Often they will look and sniff underneath as they would a mare to check any indication of a kick. I always allow the stallion to mount if he wishes, but has not dropped. Research has shown, stallions in the wild will mount numerous times before becoming erect and actually breeding. This is a natural ritual, and should not be punished. Some stallions excite themselves in this manner. When the stallion does mount, with an erection, allow him to bump the mount with his penis once or twice. This is also done in natural copulation with the mare. Then, gently deflect his penis into the AV. Never attempt to collect a stallion unless both legs are on either side of the mount. As the stallion mounts, the mare handler should walk her up a few steps. This will prevent the mare from harm by the stallion&#8217;s legs and/or teeth.</p>
<p>When training the stallion to the mount, I work in 15 to 20 minute intervals. He is then put back into his stall for 5 to 15 minutes. This is regardless of what point he is at. When he returns to the phantom, he is like a youth who has studied his homework. He wants to breed, and progresses much more rapidly. I seldom have a stallion who needs to return to the stall more then once. This too goes for any stallion who becomes aggressive. When displaying ungentlemanly behavior, they are returned to their stall, for 2 to 3 minutes, repeatedly if necessary. They quickly learn to behave in a way that earns him his reward.</p>
<p>Providing all the ingredients are present for a successful mount, ejaculation and collection, most stallions become conditioned to the memory, sight and scent stimulus, of the phantom. Many will find the stimulation of a mare unnecessary after a few collections. More important, stallion and handler are in a safe and efficient position. With the comfort of a correct phantom coupled with excellent collection technique, we have a win / win situation. A mannered stallion at ease with his job, providing you with an ejaculate of his highest quality.</p>
<p>I would like to thank EquiMount Phantoms, EquiMount@aol.com, Rancho la Rosa, and Grand World Champion Fino Stallion, Vitral, and Sabrina Gartner and her Hanoverian stallion, Royaal Z, for their help illustrating this article. Both stallions shown pictured are during their first phantom training experience.</p>
<p style="text-align: center;"><strong><a href="http://www.horsemansmall.com/ers.cfm?page=list.cfm&amp;criteria=25&amp;action=list&amp;cat=25&amp;startrow=1&amp;maxrows=10&amp;mem_ID=&amp;afid=PHJ&amp;">Click Here to Order Your Phantom!</a></strong></p>
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		<title>Ultrasound? &#8230;Ultra Practical!</title>
		<link>http://www.frozen-semen.com/site/articles/ultrasound-ultra-practical</link>
		<comments>http://www.frozen-semen.com/site/articles/ultrasound-ultra-practical#comments</comments>
		<pubDate>Fri, 13 May 2011 21:53:45 +0000</pubDate>
		<dc:creator>admin</dc:creator>
				<category><![CDATA[Articles]]></category>

		<guid isPermaLink="false">http://184.172.187.61/~frozense/site/?p=331</guid>
		<description><![CDATA[Ultrasound, as a diagnostic tool in Equine management and health care, has become staple of our industry. While the Racing and Show World utilizes ultrasound to scope everything from lungs to limbs, it is the reproductive aspects we will address here. Ultrasound technologies and their use have been with us since the late 1950s. Given [...]]]></description>
				<content:encoded><![CDATA[<p><span style="font-family: Verdana;">Ultrasound, as a diagnostic tool in Equine management and health care, has become staple of our industry. While the Racing and Show World utilizes ultrasound to scope everything from lungs to limbs, it is the reproductive aspects we will address here.</p>
<p>Ultrasound technologies and their use have been with us since the late 1950s. Given it&#8217;s recognized benefits and efficacy for medical diagnosis, it is also one of the clinically safest procedures available for both technician and patient.</p>
<p>Let us begin with how ultrasound works. The machine itself, by way of a transducer sends out beams of sound for very short periods of time, and then listens for echos. Perhaps the best example would be; Place yourself in a large, empty room. You shout and then listen. An echo is heard as sound bounces off the walls. The further away you stand from the wall, the longer it takes for the echo to return to you. You have now sent out a sound beam, and your ears are picking up the returned echo. Your brain translates this into a picture, or recognition.</p>
<p>The ultrasound machine is simulating this by using what it hears, to create a picture on a screen. Every time it hears an echo from the sound beam it has sent out, translates it onto a monitor. The location of each dot depends on how long it takes the echo to reach the machine. Gray shades are determined by how strong, or loud, the machine perceives the echo to be. An echo from a hard smooth surface will be louder then a soft bumpy surface. A strong echo comes from deeper, and is perceived and viewed as a whiter image. While a weak or soft echo, being either closer proximity or fluid, will be grey to black. The ultrasound machine sends out thousands of beams a minute in order to create the image we see on the screen. It then takes a trained technician, (vet) to read the images.</p>
<p>Tissues vary in their echocentricity, or ability to reflect sound waves. Air and dense tissue reflect most sound waves, whereas fluids propagate them. Reflected sound waves are received by the transducer and converted to electronic impulses in the machine, and displayed on the monitor.</p>
<p>The equipment consists of these three primary parts:</span></p>
<ol>
<li><span style="font-family: Verdana;"><strong>The TRANSDUCER</strong> is the component held in the examining hand of the person performing the ultrasound. Within it is a crystal that emits the ultrasonic beam, or high frequency sound wave, and then &#8220;listens&#8221; for the echoes. The echoes produce images of soft tissues and organs. The examiner moves the transducer within the area to be examined, aiming it at specific structures such as the ovaries or uterus.</span></li>
<li><span style="font-family: Verdana;"><strong>The MONITOR</strong> is simply a TV screen that receives and displays the images created. Often it has a stop / freeze capacity, and an attached Polaroid camera for capturing the images.</span></li>
<li><span style="font-family: Verdana;"><strong>The MACHINE</strong> contains the computer and power to transform the echoes the trasducer hears, to the picture you and the examiner see on the monitor. It also has a control panel and can measure structures, such as follicles.</span></li>
</ol>
<p><span style="font-family: Verdana;">There are currently two methods of performing reproductive ultrasounds, trans- abdominal and trans-vaginal.</p>
<p>The trans-abdominal method is frowned upon in equines, due to the density of the body, and conflicting organs. There are commercially available units, used by breeders to identify early pregnancies. These do not have capability or accuracy in pregnancies past 14 days, and do not detect ovulation or additional reproductive applications.</p>
<p>Trans-vaginal ultrasound is the form breeders are familiar with. After carefully removing feces from the rectal tract, an ordinary rectal palpation is done. Next, a well lubricated transducer is introduced into the rectum of the mare, and as with palpation, the organs are identified and scanned, in a systematic manner. Care is taken that no air or fecal matter is caught between the transducer and the tissue or organ of interest. It is also preferable to advance the transducer over the cervix and body of the uterus until the bifurcation of the uterus is visualized. The transducer is then moved slowly over the horn to the tip, to image the ovary. Pre-ovulatory follicles are seen as black spheres, as they are an-echoic, or fluid filled. Ovulating follicles have a pear shape or are losing their cylindrical shape. Post ovulation, the follicle becomes a corpus luteum, or yellow body, losing it&#8217;s fluid content, and are viewed as a light grey or white sphere.</p>
<p>Diagnostic ultrasonography is used in the broodmare for:</span></p>
<ol>
<li><span style="font-family: Verdana;">Evaluation of ovarian activity; Gauging stages of estrus cycle, identifying estrus, diestrus, anestrus, prediction of ovulation, &#8220;silent heat&#8221; mares.</span></li>
<li><span style="font-family: Verdana;">Detection and evaluation of pregnancy; With a high quality ultrasound unit, equipped with a 5 MHz trans-rectal transducer, embryonic vesicles can be detected as early as 9-10 days post ovulation, also twins, and estimation of conceptus age. Additionally, gender can also be determined, by identifying the position of the genital tubercle, at 60 to 70 days post ovulation.</span></li>
<li><span style="font-family: Verdana;">Diagnosis of changes and problems in the reproductive tract; These can include, but are not limited to, uterine endometrial cysts, fluid accumulations, abscesses and masses.</span></li>
</ol>
<p><span style="font-family: Verdana;">Although not a common procedure, ultrasonography in the stallion is a excellent tool for diagnostics of what otherwise would be inaccessible. It permits non invasive evaluation of the testes. This would be for both fertility problems and to estimate daily sperm output. Rectally in the stallion, it allows access to the accessory genital glands, and abdominal / inguinal exploration for chryptorchids.</p>
<p>In the growing field of Frozen Semen, success greatly hinges on the use of ultrasound. Frozen spermatazoa, post thaw, have a very short life span. Unlike fresh chilled or live cover, whom can have a 48 hour viability post ejaculation, thawed cell have a 6 to 8 hour viability. Thus timing the insemination, 8 hours prior, or 6 hours post ovulation, must be strictly adhered to. Ultrasound checks are numerous when ovulation approaches, and can admittedly be quite costly. Additionally, since there is a higher occurrence of uterine inflammatory response with frozen semen, one must check to make certain the uterus has evacuated efficiently. Often this is with the aid of Oxytocin and post insemination flushes. Uterine fluid will show up as black, since they are an-echoic, in an ultrasound exam.</p>
<p>There will repeatedly be the controversy of cost versus chance, in taking advantage of the ultrasound technology. I seem to always have the same response. Horse shows are very costly, as are trainers, advertising. trucks, trailers, show clothes, barns, stud fees, shipping, feed, and on and on. Why start short changing now. Success in a breeding program is money saved. Foals on the ground, with the least amount of effort, makes for a monitarily successful, and professional, breeding business. Certainly not all breeding endeavors require ultrasound. Yet, please consider, it can be one of the most time, labor, and overall cost saving tools you can employ.</p>
<p>Many thanks to Dr. Bob Smith, of Palm West Veterinary Hospital, Wellington Florida, for his patience, photos, and ultrasound information and expertise. Also, thanks to Lassergut Farms, USA for sharing their experience and use of their mares and facility.</span></p>
<p>&nbsp;</p>
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		<title>Breeding Soundness Exams</title>
		<link>http://www.frozen-semen.com/site/articles/breeding-soundness-exams</link>
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		<pubDate>Fri, 13 May 2011 21:52:56 +0000</pubDate>
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		<description><![CDATA[A breeding soundness exam, or BSE, is a necessary and practical expense for both breeder or buyer. They are done on both mares and stallions, and include a battery of tests to determine the breeding status of the animal, and health of the reproductive and endocrine systems. Often when purchasing a mare, the buyer will, [...]]]></description>
				<content:encoded><![CDATA[<p><span style="font-family: Verdana;">A breeding soundness exam, or BSE, is a necessary and practical expense for both breeder or buyer. They are done on both mares and stallions, and include a battery of tests to determine the breeding status of the animal, and health of the reproductive and endocrine systems.</p>
<p>Often when purchasing a mare, the buyer will, and should, do a vet check. Although the mare may not be purchased for the intended purpose of reproduction, she may be harboring reproductive problems that could impede her performance career. These may include cystic ovaries, uterine infections, chronic or otherwise, hormonal imbalances, and vaginal windsucking to name a few. For this reason, we recommend a BSE in addition to physical exam, blood work and x-rays, when investing in any mare.</p>
<p>Pre season BSEs need to be addressed as early as November for the Febuary breeder. Often when finding a manageable problem, it can take time to identify and alleviate. If waiting until February, you can lose valuable weeks of your season before your mare is &#8220;clean.&#8221; As example, if a biopsy is found to be necessary, you would not breed on that cycle, but wait for the next. Time lost can mean the decreased value of a foal born later in the season.</p>
<p>A breeding soundness exam, may consist of all, or a combination of tests. These would depend on the mare&#8217;s age, breeding status, and history. The history is quite important, and if at all possible, should be presented first, to the vet preforming the BSE. Examples of this background would include:</span></p>
<ol>
<li><span style="font-family: Verdana;">Has she been previously bred.</span></li>
<li><span style="font-family: Verdana;">How many live foals delivered.</span></li>
<li><span style="font-family: Verdana;">Any foaling problems.</span></li>
<li><span style="font-family: Verdana;">How many cycles to settle.</span></li>
<li><span style="font-family: Verdana;">Any early embryonic loss.</span></li>
<li><span style="font-family: Verdana;">Average length of estrus cycle.</span></li>
<li><span style="font-family: Verdana;">Abortions, and at what stage of pregnancy.</span></li>
<li><span style="font-family: Verdana;">Previous treatment for uterine infection.</span></li>
<li><span style="font-family: Verdana;">When was her last foal.</span></li>
</ol>
<p><span style="font-family: Verdana;">The exam commences with a visual exam of the mares vaginal conformation. This includes the vulva, perineum and anal area. As mares age, and this is certainly more prevalent with certain breeds, the vulva, normally vertical, tips, creating a recessed anus. This gives the vulva a shelf effect, and allows dropping manure to contaminate the area, in turn allowing bacteria to find entrance to the vaginal tract. The opposing lips should not sag or gap. If they do not seal, the mare will aspirate air, predisposing her to uterine infection. This condition is called Pneumovagina. A procedure called a Caslick, effectively corrects this. It is a simple suturing of the external vulvar lips. Mares in race training are routinely Caslicked when they enter training. This is to alleviate them from windsucking, common during speed work. Without the Caslick, they are predisposed to vaginal, uterine and endometrial problems, in turn, affecting performance. Caslicks are removed shortly before parturition, in the bred mare. Open mares are often Caslicked, as routine, to keep them clean until the next season.</p>
<p>Your vet will then move on to the internal exam. A rectal palpation is used to check the status of her ovaries, possible follicles, uterus, uterine tone and cervix. Pregnancy status is also confirmed. The ovaries are kidney bean shaped and can range in size from a marble to a golf ball. Hard, smooth, small ovaries are indicative of anestrus and, or a hormonal imbalance. Ovarian tumors can create an oversized ovary. The vet is looking for follicular activity, either a pre-ovulatory follicle or a corpus luteum. These would indicate normal estral activity.</p>
<p>Moving on to the uterus, which consists of a body and opposing horns, forming a Y or T formation. It is checked for size, symmetry of the horns, and abnormalities. These may include possible tumors, atrophy of the endometrial folds, or lymphatic cysts, to name a few.</p>
<p>The final area to check is the cervix. Physically it is a tube like structure that connects the vagina to the uterus. It is of vast importance to the reproductive efficiency of the mare. During diestrus, (non heat) or pregnancy, it is closed and firm. During estrus, it softens and dilates to allow passage of the ejaculate or insemination. If the mare loses the ability to close and keep the cervix sealed, she will abort. Damage to the cervix can come through difficult foal delivery, rendering tears and scar tissue. To check the cervix, the vet will additionally do a visual check, by placing a vaginal speculum into the vagina. Using a flashlight, the entire vaginal canal can be inspected. Scarring and tears to the cervix can be observed along with possible pooling of fluid or urine.</p>
<p>Specimens are now collected. A uterine endometrial culture and cytology are routine. These are done with the introduction of a swab into the uterus, and the samples grown and examined for uterine infection, and the presence of microorganisms. The cytology is a study of the actual cells for evidence of contamination, yeast, fungi, etc.</p>
<p>An endometrial biopsy is the best method of evaluating the mare as a potential broodmare. It uses a category system for prognosis.</span></p>
<table cellspacing="2" cellpadding="2" width="400">
<tbody>
<tr>
<td width="100"><span style="font-family: Verdana;">1. Class 1:<br />
2. Class 11A:<br />
3. Class 11B:<br />
4. Class 111:</span></td>
<td valign="TOP"><span style="font-family: Verdana;">80 &#8211; 90% chance of carrying to term<br />
50 &#8211; 80%<br />
10 &#8211; 50%<br />
Less then 10% chance of carrying to term</span></td>
</tr>
</tbody>
</table>
<p><span style="font-family: Verdana;">An alligator type uterine forceps, introduced vaginally, extricates a small piece of endometrial tissue. Most serious problems, that can adversely affect a mare&#8217;s fertility, can be detected only by these samples, under a microscope.</p>
<p>Additional information can be obtained with the use of transrectal ultrasound. One of the best uses of ultrasound, in a BSE, is it&#8217;s ability to &#8220;see&#8221; fluid accumulation within the uterus. Another excellent diagnostic tool is blood work. Non physical problems in mares are often the result of inefficiencies of the Endocrine system. It is these glands that are the brain of the entire reproductive factory. Without the proper hormonal stimulation, there can be reproductive shutdown. A hormonal assay is a quick, inexpensive diagram of your horses reproductive fuel source.</p>
<p>Stallions too should receive a yearly exam. As a stallion ages, he goes through physical changes. After age 13, you may see signs of testicular degeneration. Before considering a stallion for purchase, breeding, or pre season re-evaluation, a BSE would include:</span></p>
<ol>
<li><span style="font-family: Verdana;">Penile cultures, including Urethra, pre and post ejaculation, Fossa Glandis, and semen culture</span></li>
<li><span style="font-family: Verdana;">Semen collection and evaluation for Progressive Motility, Concentration, Volume, Morphology, Longevity, Velocity, Extenders</span></li>
<li><span style="font-family: Verdana;">Testing for STDs</span></li>
<li><span style="font-family: Verdana;">Testicular palpation, with ultrasound if necessary</span></li>
<li><span style="font-family: Verdana;">Check Orientation of testicles</span></li>
<li><span style="font-family: Verdana;">Daily sperm production VS Daily sperm output</span></li>
<li><span style="font-family: Verdana;">Hormone analysis</span></li>
</ol>
<p><span style="font-family: Verdana;">Additional visual observations of the BSE include; Signs of testicular degeneration, Libido, Number of mounts, Ability to maintain erection, Mount without difficulty, Physical ability to thrust, Ability to ejaculate sperm.</p>
<p>It is possible for a stallion to hold back sperm, ejaculating only seminal fluids. This is often caused by previous breeding and/or collection mismanagement, but can also be physical. Stallions who do so can become &#8220;accumulators&#8221; and develop blockages. In most cases these can be eliminated through repeated, multiple, daily collections.</p>
<p>It is unfortunate that culturing stallions at the onset of each breeding season, is not a prerequisite of breeders. Stallion owners demand clean cultures and cytologies of mares to be bred, and mare owners should do likewise. Mare owners have every right to inquire as to a stallion&#8217;s breeding soundness exam, and current status. Whether a stallion had a good past season, is not necessarily an indication of the current one. Mares may be high tech, high maintenance, factories, but stallions are not machines.</p>
<p>Annual pre season breeding soundness exams are good horse sense, good business sense, plus outstanding preventative medicine. Talk to your vet early to prepare for your coming breeding season. Discussing and implementing a program, including early breeding soundness exams, will help you realize your reproductive expectations, and put you on the road to a successful endeavor.</span></p>
<p>&nbsp;</p>
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		<title>Empty Mares &#8211; It&#8217;s a draw!</title>
		<link>http://www.frozen-semen.com/site/articles/empty-mares-its-a-draw</link>
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		<pubDate>Fri, 13 May 2011 21:52:01 +0000</pubDate>
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		<description><![CDATA[A mid summer&#8217;s nightmare for the mare owner/ breeder&#8230;empty mares. We started the last season feeling quite confident. New breeding technology articles and books were read, and we had consultations with our vet. We&#8217;ve spent hours looking through trade publications for the best stallion cross available. We&#8217;ve put the mares under lights, done cultures, cytologies, [...]]]></description>
				<content:encoded><![CDATA[<p>A mid summer&#8217;s nightmare for the mare owner/ breeder&#8230;empty mares. We started the last season feeling quite confident. New breeding technology articles and books were read, and we had consultations with our vet. We&#8217;ve spent hours looking through trade publications for the best stallion cross available. We&#8217;ve put the mares under lights, done cultures, cytologies, biopsies, palpations, and ultrasounds. Perhaps we&#8217;ve found her &#8220;dirty&#8221;, and have added flushes and oxytocin to our expense list. Let&#8217;s not forget ordering shipped or frozen semen, inseminations, and shipping charges. After many months of having our hope balloon burst&#8230;every 21 days, our frustration parameters have been met.</p>
<p>The loss is no less devastating for the single mare breeder than the large production farm. While an empty year for the hobby enthusiast means a considerable expense, with not even a tax deduction, a 20% barren rate on a breeding farm is a business disaster. Let&#8217;s take a farm with 100 broodmares. 20 mares have not settled, and must be put back to pasture for 7 months. Each have already incurred expenses, including daily care and vet, averaging over $2,000.00 for the 5 month breeding period. Add to that, It&#8217;s a draw!</p>
<p>A mid summer&#8217;s nightmare, for the mare owner/ breeder, &#8230; empty mares. We started the last season feeling quite confident. New breeding technology articles and books were read, and consultations had with our vet. We&#8217;ve spent hours looking through trade publications, for the best stallion cross available. We&#8217;ve put the mares under lights, done cultures, cytologies, biopsies, palpations, and ultrasounds. Perhaps we&#8217;ve found her &#8220;dirty&#8221;, and have added flushes and oxytocin to our expense list. Let&#8217;s not forget, ordering shipped or frozen semen, inseminations, and shipping charges. After many months of having our hope balloon burst, every 21 days, our frustration parameters have been met.</p>
<p>The loss is no less devastating for the single mare breeder than the large production farm. While an empty year for the hobby enthusiast means a considerable expense, with not even a tax deduction, a 20% barren rate on a breeding farm is a business disaster. Let&#8217;s take a farm with 100 brood mares. 20 mares have not settled, and must be put back to pasture for 7 months. Each have already incurred expenses, including daily care and vet, averaging over $2,000.00 for the 5 month breeding period. Add to that maintaining the mare for an additional 7 months and an additional $1,000.00. Multiply this by 20 mares, and we have at minimum, a $60,000.00 loss. Few businesses can continue taking hits like these. But let&#8217;s not stop here. We bought these good mares at an average of $10,000.00 each. We are now looking at a loss of over a quarter of a million dollars, that could escalate if we try and fail next year.</p>
<p>With a breeding farm, it is part of the cost of doing business. The IRS even have provisions for these losses. As &#8220;hobby&#8221; breeders, the IRS&#8217;s term for those of us who breed for profit, but slip between definition cracks, we have total emotional and monetary loss. A three horse backyard family, 4H project perhaps, getting 2 out of 3 mares in foal may not break even. This is not encouraging!</p>
<p>The fact is, no matter how I would like to give argument against the fact, under the most idyllic conditions, not all mares will become pregnant. SCIENCE MAY DICTATE, BUT MOTHER NATURE RULES! Human nature, on the other hand, is always predictable. Someone must be at fault. And we give ourselves multiple choices. Let&#8217;s start with the potency of the stallion, then the breeding manager, and/or staff. Next the air or shipping line, the vets, collector, container had been tampered with, even the weather can be blamed. Now fearing redundancy, I must repeat, perfectly timed, clean mares receiving excellent quality and correct quantity semen at the correct moment of ovulation, may not conceive or hold till ultrasound exam.</p>
<p>So far this has been a rather depressing chapter in our breeding chronicles. So let&#8217;s find a brighter scenario for this season. First, do not continue to breed your mare year round. There has been research done on mares bred in excess of three cycles, in hopes of conception. Interestingly, it was found that mares form a cycle of not conceiving. The uterus forms antibodies to the semen and conceptus. Turning her back out, or returning her to a show or pleasure life seems to improve her chances the following year. If you have lost more then one year, without finding a realistic problem, it might be wise to remove her from your breeding program. Selling or trading her is always optional. If she has great value, and it is within your budget, consider embryo transfer. Another possibility is changing stallions. Pick from a different genetic line, particularly if you were line breeding. Choose a stallion with an incredibly high conception rate, even if he was not your first choice. Often once you get your mare in foal, it is easy to repeat the success and thereby breaking her barren cycle.</p>
<p>Finally, I realize this may have no scientific backup, but I put barren mares in the pasture together with mares ready to foal and with new foals at their sides. Do not start to breed her early in the year, perhaps waiting till late March, and then breed. Allow Mother Nature to become involved. Your mares natural instincts will come into play when being in the company of the other.</p>
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		<title>Artificial Insemination (AI) &#8211; Fiction vs. Fact</title>
		<link>http://www.frozen-semen.com/site/articles/artificial-insemination-ai-fiction-vs-fact</link>
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		<pubDate>Fri, 13 May 2011 21:51:20 +0000</pubDate>
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		<description><![CDATA[The term, Artificial Insemination, or often referred to by it&#8217;s initials &#8220;AI,&#8221; has become a catch all phrase for most phases of reproduction not done by live cover. In definition, it is the process of introducing live, prepared semen, into the uterus, by means of a pipette and syringe, by a veterinarian or technician, for [...]]]></description>
				<content:encoded><![CDATA[<p>The term, Artificial Insemination, or often referred to by it&#8217;s initials &#8220;AI,&#8221; has become a catch all phrase for most phases of reproduction not done by live cover. In definition, it is the process of introducing live, prepared semen, into the uterus, by means of a pipette and syringe, by a veterinarian or technician, for the purpose of fertilizing an ova. This process is done vaginally, with the pipette passing through the cervix. The prepared dose of semen is deposited, and then makes it&#8217;s way to the ova fossa, the site of conception. When utilizing a fertile stallion, and a reproductively sound mare, both under proper and knowledgeable breeding management, conception rates are higher then live cover.</p>
<p>Why then is a large portion of the breeding public frustrated, misinformed, or shipped semen illiterate? Luckily we are beginning to see a great deal more information, being published, discussed on dedicated chat sites, and clinics and lectures, focusing on reproduction technology.</p>
<p>Aggressively educating oneself is one key to success if investing in a breeding program. Wether you are, contemplating breeding your one and only pet mare, a hobby enthusiast, or serious horse breeding business, it is an investment in time and capitol, not to mention sweat equity. The next key is utilizing proper procedures. This applies not only to yourself, but feeling confident about your prospective stallion&#8217;s breeding management. Never be concerned about asking questions. These should include:</p>
<ol>
<li>Has the stallion been evaluated and cultured at the beginning of each breeding season?</li>
<li>What is his past conception rate per cycle?</li>
<li>What methods and equipment are used to determine his motility and concentration, thereby shipping a correct dosage of 1 billion progressively motile cells (PMC)?</li>
<li>Will I receive 1 or 2 doses of 1 billion PMC in a shipment?</li>
<li>Do you ship only on specific days? Fed X or air?</li>
<li>Will he be available throughout the breeding season?</li>
<li>What is the longevity of his shipped semen?</li>
<li>Will I receive a complete report of my dose&#8217;s collections stats, including, date collected, motility, concentration, volume and extender used?</li>
<li>What are all costs involved, outside of the actual stud fee?</li>
<li>Are your doses a preferable less then 50ml?</li>
</ol>
<p><span>the other side of the coin, the stallion manager should ask of you;</span></p>
<ol>
<li>What is the mare&#8217;s breeding history?</li>
<li>Have you done a reproductive breeding soundness exam for this season, including a culture and cytology?</li>
<li>If she was &#8220;dirty&#8221; was a resistance test done for correct and best antibiotic treatment, and was a subsequent culture and cytology done?</li>
<li>Who is going to perform the insemination?</li>
<li>What method will you be utilizing to determine ordering semen; teasing, palpation, ultrasound, or a combination?</li>
</ol>
<p><span>Reasons for these questions brings us to the fiction and fact aspect of our subject.<br />
<span style="color: red;"><strong>FICTION</strong>, it only take one sperm cell to impregnate a mare.</span><br />
<strong>FACT</strong>, live cover needs 100 million progressively motile cells, on farm AI takes between 300 and 500 million, and shipped requires 1 BILLION PROGRESSIVELY MOTILE CELLS, for conception. These are internationally accepted figures.</span></p>
<p><span style="color: red;"><strong>FICTION</strong>, high motility equals high fertility.</span><br />
<strong>FACT</strong>, although no one would knock high motility and or concentration, it is not a necessity for conception in a shipped semen program. You need to be able to deduce both in order to get a 1 billion PMC dose, and this should be done by a phase contrast microscope, with a heated stage, and some form of sperm counter. So it is the final dose that counts, not the motility. I have a number of stallions we collect that have never been above 60%, with a 100 % conception rate for years. The formula is: volume x concentration x motility = # of progressively motile cells available. Let&#8217;s look at this under a couple of different scenarios.</p>
<p>Our first stallion has given us a collection of 50 ml of gel free semen. His concentration is 200 million per ml, and his motility is 80%. We take 50 x 200 million x 8o% which equals 8 billion cells. Knowing we are shipping 1 billion per dose, we know we have 8 doses available. We divide the 50mls by 8 rounding off to 6.5 ml of semen per dose, adding 33 ml extender (5 to 1), gives us a prepared dose of approximately 40 ml. Mares in research programs, have shown lower conception rates when inseminated with more than 50 ml at a time.</p>
<p>Our second stallion&#8217;s collection netted us a nearly identical 50 ml of gel free ejaculate, with a concentration of 200 million per ml, but his motility is 35 %. Big difference. But is this stallion any less fertile using correct methods of preparing his semen? We again multiply 50 x 200, but then x 35%. It equals 3.5 billion total cells. This is enough for 3 doses. Our problem now lies in the fact that to properly extend this, I would need to add 14.8 ml of raw semen to 75 ml extender, making the dose a whopping 90 ml. Quite a bit over our 50 ml. limit. We have 2 choices. First, divide into two 45 ml packets, with instructions to inseminate 4 hours apart, letting the mare owner know that two packs equal 1 dose. Second choice, and more preferable, is utilizing a centrifuge, spin the semen down, extended 1&#215;1, as one would to freeze semen, and re-hydrate your sperm pellet with 40 ml extender, giving you a strong adequate dose. One must also figure in cell loss when centrifuging. I would finish with 2 doses, of this ejaculate of 3.5 doses, utilizing this procedure.</p>
<p>Although our first stallion makes life easier for his manager, there is no difference in the quality and potential fertility of a shipment from either stallion. Additionally, either could lose an additional 50 percent of the motility, during shipping, that they began with, and still be totally viable. My personal best was a pregnancy, and resulting foal, out of 10% motility which arrived at 4%, shipped 2200 miles.</p>
<p>I can not stress enough the importance of making certain the stallion you choose for a shipped semen program, has an adequate lab, and technicians who do not cut corners. I would prefer to be told that I could not receive semen because the stallion produced 2 doses that day and I was third on the list, than to be shipped an insufficient amount, less then 1 billion cells, that compromised any efficiency of the insemination. This is a most common problem in communication and understanding between management and mare owner, and an area where time and money are forfeit.</p>
<p><span style="color: red;"><strong>FICTION</strong>, keep breeding till she is pregnant, or, the more you breed, the better your chance.</span><br />
<strong>FACT</strong>, mares set up a normal inflammatory response to seminal fluids. A healthy normal uterus flushes itself, to be rid of excess fluid. In older mares, the uterus loses it&#8217;s natural gravity causing tilt, making it a site of infection and &#8220;pooling&#8221;. For this reason, your vet might wish to flush your mare, 4 to 6 hours post insemination. All viable semen will have arrived at the ova fossa, site of conception, within 4 hours. Any cells remaining in the uterine body, are not viable, and should be flushed. An injection of Oxytocin is also indicated, to begin the contractions that flush the uterus. Research has recently proven that the uterus also builds antibodies against future semen, wether deposited naturally or through artificial insemination. It actually build an immune system to the semen after 3 cycles. Therefore, if you have bred through 3 cycles, are certain through diagnostic tests, that your mare is reproductively clean and sound, and the stallion&#8217;s collection and shipment methods are correct, stop breeding. You will be wasting money at this point. Give the mare 2 to 3 cycles off. This then also applies to breeding live cover or inseminating a mare more than twice during her cycle. Excess coverage of the mare sharply decreases chance of conception. You should by means of palpation or ultrasound, be able to determine ovulation, and should not order semen before the mare has a 35 follicle. Try to inseminate as close to ovulation as possible.</p>
<p><span style="color: red;"><strong>FICTION</strong>, discard semen if it arrives the morning the mare ovulates.</span><br />
<strong>FACT</strong>, inseminating within hours of ovulation is an excellent time. Arabian mares tend to conceive better with post ovulation insemination. There is a window though, and conception rates decline after 8 hours. Most mares ovulate between 4AM and 10AM.</p>
<p>There are no set cost to shipped semen and AI. Make certain you have asked your questions, are satisfied with the answers, and have it as part of your contract or agreement. Many stallion owners include shipped semen and it&#8217;s cost in the stud fee. If an outside veterinarian or lab is used, and there is an additional cost to you, do not be shy about contacting them for answers. Always discuss your plan with your own veterinarian, and set up a program for pre breeding reproductive health exam, and check out his/her background and availability as to artificial insemination, procedure, and disposables. It is preferable to use non spermicidal syringes and lubricant. This would give him/her time to prepare. Know the charges you may incur for the exam, flush, insemination, ultrasounds, etc. Being surprised is never pleasant.</p>
<p>Fiction belongs on your bookshelf. As an informed breeder utilizing the outstanding technologies available to us, the fact is, success is an odds on favorite.</p>
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		<title>Cryopreservation&#8230;Freezing Semen</title>
		<link>http://www.frozen-semen.com/site/articles/cryopreservation-freezing-semen</link>
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		<pubDate>Fri, 13 May 2011 21:46:48 +0000</pubDate>
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		<description><![CDATA[Equine Cryopreservation is the process of collecting, processing, freezing and storing a stallion&#8217;s genetic material. It is perhaps the most misunderstood and therefore backed away from technology in advanced equine reproduction. Research is continuing at numerous universities and private enterprises to advance our current knowledge, and smooth out what has become recurring problems. At this [...]]]></description>
				<content:encoded><![CDATA[<p>Equine Cryopreservation is the process of collecting, processing, freezing and storing a stallion&#8217;s genetic material. It is perhaps the most misunderstood and therefore backed away from technology in advanced equine reproduction. Research is continuing at numerous universities and private enterprises to advance our current knowledge, and smooth out what has become recurring problems.</p>
<p>At this time, conception rates with frozen semen remain at about 50%. Even with this, there are many critical factors for success. There must be proper collection techniques, with a close to sterile environment. The stallion should have a progressive motility, upwards of 50% (bare minimum). Middle to high concentration is also preferred. Many aged stallions, beyond 13 years, while developing varying degrees of testicular degeneration, produce larger volumes of fluid with less sperm cells. It is from this group we receive the most requests for freezing. Owners of young, virile stallions are not as concerned at the moment for saving their stallions genetic material, thus insuring their future. Often when we are called, it is too late. Many aged stallions can continue to be successful with live cover, and even ship well with the aid of a centrifuge, but lose potential to freeze enough straws per collection, to make it economically feasible. It is therefore imperative to decide on banking semen while the stallion is in his prime, if circumstances permit. Many stallions are not suitable at any age. Young stallions may exhibit poor seminal quality due to other factors such as: medications, physical condition, nutrition, over breeding, testicular size, overwork, and heredity, to name a few.</p>
<p>The freezing process begins similarly to a normal collection. We are using some form of sperm counter to assess concentration, and preferably a phase contrast microscope with a heating stage, centrifuge, incubator, liquid nitrogen tank, and waterbath.</p>
<p>We start by washing the stallion thoroughly, making certain he is dry. If using anything other than warm water for cleaning, it is imperative to rinse thoroughly, making certain there is no residue. I recommend doing this type of washing, the day before collection, and washing with warm water only, prior to collection. A mild detergent such as Ivory works quite well for removing smegma, as well as the sheath cleaning product, Excaliber. Remember, be gentle. Do not force or pick off scales. Excaliber can be left on for an hour, and when you return to finish the job, the scales are easily washed away, leaving the penis non-irritated. And always, rinse, rinse, rinse, and gently towel dry.</p>
<p>After collecting, the ejaculate is evaluated as to concentration and motility, and extended in a centrifuge medium. Centrifugation time varies, by the individual animal&#8217;s ejaculate. A good base to start at is 10 minutes at 400 G force. The centrifuge spins at extreme speed, separating the liquid from the cells. The liquid is then aspirated off leaving a sperm pellet on the bottom. It is important to leave a small amount of seminal fluid, as this appears to be necessary to the cells survival. The new concentration is then re calibrated by means of a hemocytometer, and re extended in a freezing medium. This freezing extender is a base extender with the addition of glycerol, egg yolk, and a food detergent, Equex.</p>
<p>Straws are then filled depending on their size. If macro straws, 5 ml., are used, they normally will accommodate 600 to 800 million progressively motile cells per straw, or 1 dose. Half ml. straws are also common, but take 3 or as many as 10 straws, to support a dose. Straws are then placed on a freezing rack set just above a level of liquid nitrogen. This allows the straws to initially freeze in the vapors, producing less shock. After 20 minutes they are placed directly into the liquid nitrogen which completes the freezing process. ½ ml straws may take less time in the vapor. The straws can now be transferred into a liquid nitrogen tank for storage. Properly cared for these will last forever. Regular checks and maintenance of the tanks are a priority.</p>
<p>In evaluating frozen semen, we can only test for post thaw motility. Unfortunately, this does not mean fertility. Even very high motility is no guarantee for conception. One of the problems being researched is that freezing tends to destroy 1) the cells abilities to adhere to the egg, and, 2) the enzymes needed to penetrate it, for capacitation and conception to occur. At this time there is no test to confirm or deny wether any damage has occurred, but research is currently being conducted to produce a test. Breeding a reproductively sound mare, with a sampling of your straws is recommended. A positive pregnancy test is your only warrantee. If purchasing straws, always ask if they have had a successful test, and for numbers and references.</p>
<p>The sale and purchase of equine frozen semen, is also a new avenue for most breeders. Frozen semen can be banked by the purchaser for any length of time, including years. Live foal guarantees become difficult to promise, if not impossible. The added expense of freezing and banking, is usually compensated in the breeding fee, by being paid for up front, in full. The straws are then released to the purchaser. Most stallions offering frozen semen also limit the amount of straws, or cycles, of the breeding. If the mare has not settled, additional straws can be obtained at a pre arranged cost. There are also stallions, including some deceased, that charge another full stud fee, should the mare not conceive on the allotted straws. There are pros and cons on both sides. Considerable time and cost are engaged by the stallion owner, yet the mare owner can pick and choose the right moment for insemination. The cost of shipping, and the dry shippers themselves, specific nitrogen containers for shipping, are costlier then shipping fresh chilled semen. But, you don&#8217;t have to worry about timing your mare&#8217;s ovulation. The straws are available whenever you choose, and your mare is ready.</p>
<p>Always get thaw instructions, specific for the straws you have purchased, and a contact number for the company who froze the semen. Only a few degrees, or 2 to 3 seconds, too long or too short, will damage or destroy the cells. Competent companies, with positive pregnancy results, are happy to give references. Always discuss the purchase of frozen semen straws with your veterinarian. He or she may not be experienced with working with frozen. It also is necessary to have a liquid nitrogen tank available to either yourself or your vet to store the straws. A waterbath, and straw forceps are also necessary to handle and thaw.</p>
<p>Insemination with frozen has it&#8217;s own specific needs. The mare must be carefully observed during her estrus. Monitored by ultrasound throughout, and at least twice daily after reaching a 35 ml. follicle. Ultrasound needs to be utilized every 6 to 8 hours, nearing ovulation, in order to decide the closest point, prior or post ovulation.. Most straws need to be administered within 6 hours of ovulation. The ultrasound gives us a clear picture of the follicle beginning to lose shape, just prior to ovulating. With most mares, ovulation occurs between 4 AM and 10 AM. This can be an added burden on your veterinarian&#8217;s time. Semen previously frozen, does not have the life span of fresh chilled, within the mare. Thus the need for insemination within minutes of thaw, and perfect timing of the ovulation.</p>
<p>A new protocol was presented this year at the International Reproduction Symposium at Solvang, California. It has been researched and found effective, and simplifies the insemination timing. The mare is observed at the start of estrus, and when ultrasounded at a 35 follicle, larger for certain breeds, HcG is administered. The mare is then inseminated at 24 and 40 hours post HcG. One can then determine once a day, utilizing ultrasound, the progression of her ovulation. This will definitely cut cost to the mare owner.</p>
<p>It is actually the European, Iberian, and South American markets, that have pursued the American breeders to become more adept in the use and marketing of Equine Cryogenics. In Europe, the use and shipment of straws, are as commonplace as fresh chilled is to us. For 20 plus years it has been an accepted and successful practice. Recently they lobbied the American Quarter Horse Association, so successfully, that at the AQHA convention, they passed the usage of frozen semen, beginning in 2001. Because of international customs regulations, fresh chilled was often unable to be delivered within 48 hours, diminishing sperm quality severely, if not entirely. To maintain a global genetic bank, readily available and accessible, their reasoning won out.</p>
<p>This is also an excellent way to secure healthy semen, for both exportation and importation, while also expanding your client base, or, as a mare owner, expanding your genetic pool. Each country has their own requirement for the importation of horse semen. A quarantine is generally needed, along with a health certificate. The stallion while quarantined, may use that time to be frozen. This ranges from 30 to 60 days. A quarantine may be arranged at your own barn, a specific reproductive facility or University, through your vet and the USDA. Semen frozen within the USA and shipped within the country does not require a quarantine.</p>
<p>Heredity is a severe problem we have created in the industry. In cattle, regardless of quality, show career or breeding, a reproductively inferior animal is eliminated. As horse breeders we continue to breed both stallions and mares with poor reproductive histories. Using the advancements of veterinary science, we make up for what nature has tried to cull. The result is a growing number of reproductively defective, yet outstanding, show horses. Perhaps we need a wake up call to breeders, owners, and horsemen, to recognize our responsibility to perpetuate our breeds. Our standard must be as high for producing reproductive efficiency as it is for creating an otherwise athletically and physically, superior specimen.</p>
<p>Finally, the future of our industry to grow and be financially healthy, on a global basis, is the utilization of the many advanced reproductive techniques, such as freezing, embryo and oocyte transfer, gender selection and frozen embryos. We as responsible horsemen, must continue to educate ourselves, ask questions, and select our breeding horses responsibly.</p>
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